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KMID : 0545120140240111510
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Journal of Microbiology and Biotechnology
2014 Volume.24 No. 11 p.1510 ~ p.1515
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Ovicidal Activity of Lactic Acid Produced by Lysobacter capsici YS1215 on Eggs of Root-Knot Nematode, Meloidogyne incognita
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Lee Yong-Seong
Kyaw Wai Naning
Xuan Hoa Nguyen
Kim Sun-Bae
Moon Jae-Hak
Kim Kil-Yong
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Abstract
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Lysobacter capsici YS1215 isolated from soil previously showed nematicidal potential for biological control of the root-knot nematode. In this study, lactic acid, a nematicidal compound, was isolated from culture filtrate of YS1215, and its ovicidal activity was investigated. Purification and identification of lactic acid were performed by a series of column chromatographies and identified by 1H and 13C NMR spectra and GC-MS analysis. Our results showed that bacterial culture filtrate containing lactic acid significantly inhibited egg hatching. The lowest egg hatch rate (5.9%) was found at a high concentration (25 μl/ml) of lactic acid at 5 days after incubation, followed by 20 (15.2%), 15 (23.7%), 10 (29.8%), and 5 (36.4%) μl/ml, while egg hatching in the control (sterile distilled water) was 44.5%. This is the first report of lactic acid as an ovicidal compound, and it may be considered as an alternative of chemical pesticide against root-knot nematodes.
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KEYWORD
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Lactic acid, Lysobacter capsici YS1215, bacterial culture filtrate, crude extract, egg hatch
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